Mountain West · Cortisol AM & PM Test – Cortisol Levels
Cortisol AM & PM Test – Cortisol Levels in Colorado
This page explains the cortisol am & pm test – cortisol levels, what it measures, and how to order it. A Cortisol AM & PM Test measures the stress hormone cortisol at two points in the day to show how its levels rise and fall. This pattern can help a clinician evaluate adrenal and pituitary function.
Lab requirements in Colorado
Here is what you need before ordering and completing the Cortisol AM & PM Test – Cortisol Levels in CO. Review each item and confirm the details with the collection lab.
- Physician's order
- Included. A physician's order is required for lab testing and is provided for you by our partner's physicians — no referral from your own doctor is needed.
- Fasting
- Fasting is required for this test — follow the collection lab's instructions.
- Preparation
- Preparation can vary, so follow the collection lab's instructions about timing, fasting, and any medications. Tell the lab about all prescription and over-the-counter drugs, since some can…
- Sample & visit
- A blood and/or urine sample is collected; the visit usually takes about 10 minutes.
- Identification
- Bring the lab requisition form from your account. The collection lab may also ask for a government-issued photo ID.
- Turnaround
- 1-3 business days
- Availability
- Our partner lab does not serve ND, RI, NY, NJ, MD or HI. Confirm availability for your location with the collection lab.
Before you go
- Order your test before visiting a lab, then use the requisition form in your account.
- Print the requisition form, or fax it to the lab directly from your account.
- No scheduling is needed — visit the collection lab during its normal business hours.
- A blood and/or urine sample is collected; the visit usually takes about 10 minutes.
- Bring a government-issued photo ID in case the lab requests it.
- Results are posted to your secure account within 1–3 business days.
Requirements are summarized from our partner lab's published policies. Preparation and availability can change — confirm current instructions with the collection lab before your visit.
What this test is
Cortisol is a hormone made by the adrenal glands that helps regulate energy, blood sugar, blood pressure, and the body's response to stress. Because cortisol normally peaks in the early morning and drops later in the day, testing it twice provides a more complete picture than a single measurement. This test is typically done on blood samples collected in the morning and again in the afternoon.
What it measures
- Morning cortisol — reflects the expected peak level of cortisol after waking
- Afternoon cortisol — shows whether cortisol falls later in the day as expected
- Diurnal variation — the difference between morning and afternoon levels, which reflects the normal daily rhythm
What the test involves
The test involves two standard blood draws, usually from a vein in the arm, one in the morning and one in the afternoon. Each sample is collected into a tube and sent to a laboratory for analysis.
Why it is often ordered
A clinician may order this test when symptoms such as fatigue, weight changes, high blood pressure, or mood shifts suggest a possible problem with cortisol production or regulation. It can also be used to monitor a known adrenal or pituitary condition or to follow up on abnormal results from other tests.
Preparation
Preparation can vary, so follow the collection lab's instructions about timing, fasting, and any medications. Tell the lab about all prescription and over-the-counter drugs, since some can affect cortisol levels.
How results are reported
Results are reported as cortisol values for each collection time, often with a note about whether the pattern is typical or not. Reference ranges differ between laboratories and depend on the assay and collection times, so results should always be interpreted using the range provided by the testing lab.
Before you go
- The exact times your morning and afternoon samples should be collected
- Whether any fasting or medication changes are required before the test
- How and when you will receive your results
Cities
Cortisol AM & PM Test – Cortisol Levels in Colorado communities
Choose your city for local context and to order this test.